By Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde (auth.), Edwin Yunhao Gong (eds.)
Although antiviral medicines were effectively constructed for a few viral ailments, there is still a transparent, unmet clinical have to advance novel antiviral brokers for the keep watch over and administration of many viruses that presently haven't any or constrained remedies in addition to a necessity to beat the restrictions linked to the prevailing antiviral medications, comparable to antagonistic results and emergence of drug-resistant mutations. the second one variation of Antiviral equipment and Protocols features:
- All chapters are new and written by way of specialists within the box, reflecting the foremost contemporary technical advances in antiviral learn and discovery.
- This variation makes a speciality of many very important human viruses, corresponding to human immunodeficiency virus sort 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human breathing syncytial virus (RSV), and influenza virus, whereas additionally that includes a few vital rising viruses, comparable to dengue virus, West Nile virus, and chikungunya virus.
- As a quantity within the hugely winning Methods in Molecular Biology sequence, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and pointers on troubleshooting and keeping off recognized pitfalls.
Comprehensive and state of the art, Antiviral tools and Protocols, moment Edition will function an outstanding laboratory reference for pharmaceutical and educational biologists, medicinal chemists, and pharmacologists in addition to for virologists within the box of antiviral study and drug discovery.
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Additional info for Antiviral Methods and Protocols
Add 10 μL (PR–RT) or 5 μL (RT–IN) of Ultrapure™ DNase/RNase-free distilled water to the dedicated wells for outer PCR-negative controls and outer mix control. 10. Seal the plate with sealing tape. 11. Place the PCR plate in the thermocycler. Genotyping of the HIV-1 PR- RT and IN Genes 47 12. Conduct the PCR using the following reaction conditions: PR–RT: Initiate the reaction with a reverse transcription step for 30 min at 53 °C, followed by a denaturation step for 2 min at 94 °C. Continue with 40 cycles of denaturation at 94 °C for 15 s, annealing at 55 °C for 30 s, and elongation at 68 °C for 150 s.
MicroAmp® Optical 384-Well Reaction Plate (Applied Biosystems, Cat. No. 4343370). 3. MicroAmp® Optical Adhesive Film (Applied Biosystems, Cat. No. 4311971). 4. 2 mL Eppendorf tubes. 5. Transparent 384-well plates. 6. Reagent reservoirs. 6 Equipment 1. Humidified 37 °C incubator. 2. Water-bath 37 °C. 3. Heating system 95 °C with removable blocks. 4. Multidrop combi reagent dispenser with dispensing cassettes (Thermo Scientific). 5. Multichannel pipettes. 6. ABI7900HT fast real-time PCR system (Applied Biosystems).
Kilby JM, Hopkins S, Venetta TM et al (1998) Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41- mediated virus entry. Joly V, Jidar K, Tatay M et al (2010) Enfuvirtide: from basic investigations to current clinical use. Chan DC, Fass D, Berger JM et al (1997) Core structure of gp41 from the HIV envelope glycoprotein. Cell 89:263–273 10. Lu M, Blacklow SC, Kim PS (1995) A trimeric structural domain of the HIV-1 transmembrane glycoprotein. Nat Struct Biol 2:1075–1082 11.
Antiviral Methods and Protocols by Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde (auth.), Edwin Yunhao Gong (eds.)