By Leda R. Castilho
Advent to animal mobile expertise /, Animal cells: uncomplicated strategies /, Cloning and expression of heterologous proteins in animal cells /, mobile metabolism and its keep watch over in tradition /, tradition media for animal cells /, Post-translational amendment of recombinant proteins /, Mechanisms of mobilephone proliferation and mobile demise in animal cellphone tradition in vitro /, Mathematical types for development and product synthesis in animal phone tradition /, Bioreactors for animal cells /, tracking and keep an eye on of mobile cultures /, Animal telephone separation /, Product purification techniques /, qc of biotechnological items /, Regulatory elements /, highbrow estate /, Recombinant healing proteins /, Monoclonal antibodies /, Viral vaccines: recommendations, rules, and bioprocesses /, Bioinsecticides /, mobilephone remedies and stem cells /, Gene remedy /; Paula Marques Alves, Manuel Jose Teixeira Carrondo, & Pedro Estilita Cruz --, Patricia Leo ... [et al.] --, Mariela Bollati-Fogolin & Marcelo A. Comini --, Paola Amable & Michael Butler --, Angela Maria Moraes, Ronaldo Zucatelli Mendonca, & Claudio Alberto Torres Suazo --, Michael Butler --, Maira Peixoto Pellegrini, Rodrigo Coelho Ventura Pinto, & Leda dos Reis Castilho --, Elisabeth F.P. Augusto, Manuel F. Barral, & Rosane A.M. Piccoli --, Ernesto Chico Veliz, Gryssell Rodriguez, & Alvio Figueredo Cardero --, Aldo Tonso --, Leda dos Reis Castilho & Ricardo de Andrade Medronho --, Angela Maria Moraes, Leda Castilho, & Sonia Maria Alves Bueno --, Marina Etcheverrigaray & Ricardo Kratje --, Maria Teresa Alves Rodrigues & Ana Maria Moro --, Ana Cristina Almeida Muller & Leila Costa Duarte Longa --, Maria Candida Maia Mellado & Leda dos Reis Castilho --, Wirla M.S.C. Tamashiro & Elisabeth F.P. Augusto --, Isabel Maria Vicente Guedes de Carvalho Mello ... [et al.] --, Marcia Regina da Silva Pedrini & Ronaldo Zucatelli Mendonca --, Hamilton da Silva Jr. & Radovan Borojevic --, Celio Lopes Silva ... [et al.]
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Extra info for Animal cell technology: from biopharmaceuticals to gene therapy
G. nerve cells, ﬁbroblasts, Caco2, MRC-5, and endothelial cells). g. embryonic and adult stem cells). Primary cells are isolated directly from organs or tissues. Primary cells are normally heterogeneous and better represent the tissue from which they originate. These cells have a ﬁnite growth capacity and can be subcultured for only a limited number of passages. Subcultured cells, which have been selected to form a population of cells of a single type, are designated cell lines, and can be ﬁnite or continuous.
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This is the case of the so-called DNA vaccines, where an immunogenic protein begins to be produced by the host organism and, thus, a situation similar to the case of an infection occurs (Whalen, 1996). This type of vaccine is discussed more extensively in Chapter 18. For gene therapy, hematopoietic stem cells are the most common targets due to their capacity for expansion and differentiation. The current applications focus essentially on diseases caused by a localized genetic deﬁciency. , 1993).
Animal cell technology: from biopharmaceuticals to gene therapy by Leda R. Castilho