Kan Wang's Agrobacterium Protocols, Second Edition: Volume I (Methods PDF

By Kan Wang

ISBN-10: 1588295362

ISBN-13: 9781588295361

ISBN-10: 1597451304

ISBN-13: 9781597451307

A optimum selection of Agrobacterium-mediated transformation recommendations for cutting-edge plant genetic engineering, practical genomic research, and crop development. quantity 1 info the main up-to-date ideas to be had for twenty-six plant species drawn from cereal plants, business crops, legume crops, and vegetable vegetation, and offers a number of tools for introducing DNA into 3 significant version plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors additionally define the elemental tools in Agrobacterium manipulation and techniques for vector building. quantity 2 includes one other thirty-three confirmed thoughts for root vegetation, turf grasses, woody species, tropic crops, nuts and end result, decorative crops, and medicinal vegetation. extra chapters supply equipment for introducing DNA into non-plant species, akin to micro organism, fungi, algae, and mammalian cells. The protocols stick to the profitable tools in Molecular Biology™ sequence structure, every one supplying step by step laboratory directions, an creation outlining the foundations in the back of the process, lists of the required apparatus and reagents, and pointers on troubleshooting and warding off identified pitfalls.

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Extra resources for Agrobacterium Protocols, Second Edition: Volume I (Methods in Molecular Biology Vol 343)

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Then the binary vector system was invented, exploiting the fact that the process for transfer of T-DNA is active even if the virulence genes and the T-DNA are located on separate replicons in an A. tumefaciens cell (2). An artificial T-DNA is constructed within a plasmid that can be replicated in both A. tumefaciens and E. coli. Plasmid construction is completed in E. coli, and simple transfer of the vector to A. tumefaciens produces a strain ready for plant transformation. Soon such binary vectors were widely distributed among plant scientists.

1975) The genus Agrobacterium and plant tumorigenesis. Annu. Rev. Microbiol. 29, 377–405. 2. Allen, O. N. and Holding, A. J. (1974) Genus II. Agrobacterium, in Bergey’s Manual of Determinitive Bacteriology, 8th ed. (Buchanan, R. E. and Gibbons, N. ), Williams & Wilkins, Baltimore, pp. 264–267. 3. Kerr, A. (1992) The genus Agrobacterium, in The Prokaryotes, Vol. , Truper, H. ), SpringerVerlag, New York, pp. 2220–2235. 4. Snell, J. J. S. (1984) General introduction to maintenance methods, in Maintenance of Microorganisms: A Manual of Laboratory Methods (Kirsop, B.

Researchers often find undesired aspects of vector configuration when characterizing progeny of transgenic plants; in a worst case scenario these vector defects could ruin the entire project. In this chapter, steps from designing of vectors through preparation of strains of Agrobacterium tumefaciens ready for infection are addressed. It should be emphasized that good management of these processes can save a lot of precious time and resources. Basic frameworks of the current vectors for transformation of higher plants were developed in the early and mid-1980s, soon after it had been elucidated that crown gall tumorigenesis represented the genetic transformation of plant cells (1).

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Agrobacterium Protocols, Second Edition: Volume I (Methods in Molecular Biology Vol 343) by Kan Wang

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