By Frank J. Dixon (Eds.)
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Additional info for Advances in Immunology, Vol. 47
1987; Defrance et a l . , 1987). In the rodent systems incubation of B cells with 3-5 U/ml of IL-4 increased the density of FccRIIs on the majority of B cells and enhanced the release of the 38-kDa fragments of the receptors which lacked affinity for IgE. However, culture of normal mouse spleen cells or B cells with IL-4 did not contain a detectable amount of IgE-BFs, as determined by rosette inhibition (Adachi et a l . , 1988). , 1987), and enhanced the release of the 25-kDa fragments of FccRII which had affinity for IgE.
The protocol of the experiments is shown in Fig. 4. BDFl mice were immunized with alum-absorbed OVA for the IgE antibody response, and their spleen cells were obtained 2 weeks after the immunization, when the IgE antibody titer had reached maximum. The spleen cells were cultured with OVA to activate antigen-primed T cells, and the activated T cells were propagated by IL-2 in the presence or absence of GIF for 4 days. Since antigen-specific T cells should have selectively proliferated during the culture, the cells recovered from the cultures were stimulated with OVA-pulsedsyngeneic macrophages.
And Ishizaka, K. J. Zmmunol. 128, 289. Yokota, A . , Barsumian, E. , and Kishimoto, T. (1988). ) 55, 611. Young, M. , Leung, C. Y. , and Geha, R. S. (1984). Eur. J. Immunol. 14, 871. Young, M. , Geha, R. , Maksad, K. , a n d h u n g , D. Y. M. EurJ Zmmunol. 16, 985. , Barsumian, E. , Hardy, R. , and Kishimoto, T. J. Immunol. 138, 2576. , and Green, D. R. J. Zmmunol. 140, 1351. Zuraw, B. , and Katz, D. H. J. Zmmunol. 127, 1169. This article was accepted for publication on 27 February 1989. ADVANCES 1N IMMUNOLOGY, VOL.
Advances in Immunology, Vol. 47 by Frank J. Dixon (Eds.)