Download PDF by Frank J. Dixon (Eds.): Advances in Immunology, Vol. 47

By Frank J. Dixon (Eds.)

ISBN-10: 012022447X

ISBN-13: 9780120224470

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1987; Defrance et a l . , 1987). In the rodent systems incubation of B cells with 3-5 U/ml of IL-4 increased the density of FccRIIs on the majority of B cells and enhanced the release of the 38-kDa fragments of the receptors which lacked affinity for IgE. However, culture of normal mouse spleen cells or B cells with IL-4 did not contain a detectable amount of IgE-BFs, as determined by rosette inhibition (Adachi et a l . , 1988). , 1987), and enhanced the release of the 25-kDa fragments of FccRII which had affinity for IgE.

The protocol of the experiments is shown in Fig. 4. BDFl mice were immunized with alum-absorbed OVA for the IgE antibody response, and their spleen cells were obtained 2 weeks after the immunization, when the IgE antibody titer had reached maximum. The spleen cells were cultured with OVA to activate antigen-primed T cells, and the activated T cells were propagated by IL-2 in the presence or absence of GIF for 4 days. Since antigen-specific T cells should have selectively proliferated during the culture, the cells recovered from the cultures were stimulated with OVA-pulsedsyngeneic macrophages.

And Ishizaka, K. J. Zmmunol. 128, 289. Yokota, A . , Barsumian, E. , and Kishimoto, T. (1988). ) 55, 611. Young, M. , Leung, C. Y. , and Geha, R. S. (1984). Eur. J. Immunol. 14, 871. Young, M. , Geha, R. , Maksad, K. , a n d h u n g , D. Y. M. EurJ Zmmunol. 16, 985. , Barsumian, E. , Hardy, R. , and Kishimoto, T. J. Immunol. 138, 2576. , and Green, D. R. J. Zmmunol. 140, 1351. Zuraw, B. , and Katz, D. H. J. Zmmunol. 127, 1169. This article was accepted for publication on 27 February 1989. ADVANCES 1N IMMUNOLOGY, VOL.

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Advances in Immunology, Vol. 47 by Frank J. Dixon (Eds.)

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